Strains are the foundation for growing edible fungi, and their quality directly impacts both the yield and the quality of the final product. Proper preservation of these strains is crucial for ensuring consistent growth and maintaining the income of mushroom farmers. One of the most common and cost-effective methods used at home is storing strains in a household refrigerator. This method not only helps inhibit strain growth but also delays aging, making it an ideal choice for many small-scale growers. However, due to the presence of various foods like fruits, drinks, meat, and eggs in the same refrigerator, there's a risk of contamination from harmful microorganisms. Therefore, extra care must be taken when using a domestic fridge for bacterial preservation. Even though the temperature inside a household refrigerator is low, it doesn't completely eliminate the risk of infection. While high-temperature pathogens struggle to survive, some low-temperature or cold-adapted microorganisms can still thrive. Additionally, certain heat-resistant bacteria may gradually adapt to temperatures between 4–6°C, becoming potential sources of contamination for edible fungi. At the same time, other pathogenic microbes from food items entering the fridge can mix with the strains. For example, if Pseudomonas species are present, they can cause oyster mushroom rust, which becomes more severe the longer the strains are stored in such conditions. Moreover, the high humidity in the freezer section of the fridge creates a favorable environment for microbial growth, increasing the risk of infection. To prevent contamination during strain storage, farmers can employ several effective techniques. First, using paraffin wax to seal the culture tubes is a simple yet efficient method. After placing the cotton plug tightly into the tube, melt the wax and pour it over the top, allowing it to cool and form a protective layer. This prevents moisture and air from entering, reducing the chances of contamination. Second, sealing the cultures in plastic bags is another practical approach. Place the test tubes or strainer bottles inside a sealed plastic bag, and if possible, use a vacuum pump to remove air for better preservation. This method significantly improves the longevity and quality of the strains. Third, regular cleaning and disinfection of the refrigerator is essential. Clean the interior with a mild detergent and a damp cloth to remove dirt and odors, then use a negative ion generator or UV lamp for thorough sterilization. Avoid using chemical disinfectants like formaldehyde or sulfur, as they can damage the strains and leave harmful residues. Lastly, placing a desiccant in the freezer compartment helps reduce humidity, limiting the growth of harmful microorganisms and protecting the strains from damage. By following these simple steps, farmers can ensure the health and viability of their fungal cultures while minimizing the risk of contamination. Stair Railings,Stainless Steel Stair Railings,Stair Guardrails,Aluminium Zinc Steel Stair Railing Xuzhou Guifeng Metal Technology Co., Ltd , https://www.guifengmetal.com
Refrigerator Preservation Bacteria 4 items