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Flower tissue culture requirements for laboratories and equipment
Flower tissue culture involves growing flowers in a controlled, artificial environment. It is a modern, factory-like method of producing high-quality flowers, which requires specific laboratory setups and advanced equipment to ensure success.
**Laboratory Requirements**
1. **Chemical Laboratory**: This area is responsible for preparing the nutrient media used in plant cultivation. It needs various chemical reagents, different types of glassware, and precision weighing instruments to ensure accurate measurements.
2. **Washing Room**: The washing room is where all the glassware is cleaned. It should be equipped with a tap water system and an oven for drying the cleaned materials after washing.
3. **Sterilization Chamber**: This is where the culture medium and equipment are sterilized. An autoclave is essential, along with a power source and water supply to maintain proper sterilization conditions.
4. **Inoculation Room**: This is the critical area where plant materials are cut, sterilized, and inoculated into the culture medium. The room must be clean, well-ventilated, and equipped with UV lamps for regular disinfection. Some labs use an inoculation box or a laminar flow hood as an alternative.
5. **Cultivation Room**: Here, the plant tissues grow under controlled environmental conditions. The room should be clean, well-insulated, and have stable temperature and humidity levels to support healthy growth.
**Essential Equipment**
1. **Balance**: Used for measuring chemicals and hormones when preparing the culture medium. A standard balance is sufficient for most elements, while an analytical balance is needed for trace elements and growth regulators.
2. **pH Meter**: This device measures the acidity or alkalinity of the culture medium to ensure it is suitable for plant growth.
3. **Autoclave**: Essential for sterilizing the culture medium and all equipment used in the process, ensuring no contamination occurs.
4. **Oven**: Used to dry and sterilize glassware after washing, providing an additional layer of cleanliness.
5. **Distilled Water Unit**: Produces purified water necessary for making the culture medium and maintaining sterile conditions.
6. **Refrigerator**: Stores mother stock solutions and plant materials at the right temperature to preserve their quality.
7. **Inoculation Box or Clean Bench**: Provides a sterile workspace for handling plant materials during inoculation or transfer.
8. **Air Conditioner**: Helps regulate the temperature in the cultivation room, ensuring a stable environment for plant growth.
By setting up a well-equipped and organized laboratory, flower tissue culture can be carried out efficiently and effectively, leading to high-quality plant production. Each component plays a crucial role in maintaining sterility, accuracy, and optimal growth conditions.